Journal: Journal of Translational Autoimmunity

Article Title: T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis

doi: 10.1016/j.jtauto.2025.100345

Figure Lengend Snippet: RA-Specific Serum IgG Antibodies in DBA/1J Mice. Sera were collected biweekly from DBA/1J mice immunized with bovine type II collagen (bCII) (arthritic: N = 9–18; non-arthritic: N = 17–37), or PBS (N = 14) and tested via indirect ELISA for A) anti-CitP, and B) anti-HomoCitP IgG antibodies. Graphs show the median [IQR] for each group, with a cut-off indicating the limit of detection (dashed line). Statistical analysis was performed using a mixed effects model with the Geisser-Greenhouse correction and Tukey's multiple comparisons test. The resulting p-values are indicated on the graphs.

Article Snippet: The anti-mCII ELISA kits were used according to manufacturer's protocol (2036T; Chondrex).

Techniques: Indirect ELISA

Journal: Frontiers in Immunology

Article Title: MSC-derived microvesicles inhibit the progression of arthritis in a murine model of preclinical rheumatoid arthritis

doi: 10.3389/fimmu.2026.1723572

Figure Lengend Snippet: Increased Expression of Serum Pro-Inflammatory Factors in Pre-CIA Mice. (A) ELISA was employed to measure the serum levels of key inflammatory markers and autoantibodies in the saline, Pre-CIA, and CIA groups. (B) interleukin-1β (IL-1β), (C) tumor necrosis factor-α (TNF-α), (D) interleukin-10 (IL-10), (E) interleukin-6 (IL-6), (F) interleukin-17a (IL-17a), (G) C-reactive protein (CRP), (H) anti-cyclic citrullinated peptide (anti-CCP) antibodies, and (I) anti-collagen type II (anti-CII) antibodies. The Pre-CIA group exhibited significantly higher levels of pro-inflammatory cytokines (IL-1β, TNF-α, IL-6, IL-17a, and CRP) compared to the saline group, but the levels of pro-inflammatory cytokines in the Pre-CIA group were lower than those in the CIA group. The anti-inflammatory cytokine IL-10 and autoantibody levels (anti-CCP and anti-CII) exhibited a similar trend, reflecting an intermediate inflammatory state in the Pre-CIA group. Data are expressed as mean ± SD. Statistical comparisons were conducted using one-way ANOVA followed by Tukey’s post-hoc test. Significance levels were defined as two-tailed p < 0.05, with *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, and N.S. indicating not significant.

Article Snippet: The serum concentrations of tumor necrosis factor alpha (TNFα, Abcam #ab208348), interleukin-1 beta (IL-1β, Abcam #ab197742), interleukin-17A (IL-17A, Abcam #ab199081), interleukin-10 (IL-10, Abcam #ab255729), C-reactive protein (CRP, Abcam #ab222511), interleukin-6 (IL-6 Abcam #ab222503), anti-cyclic citrullinated peptide antibody (anti-CCP, CUSABIO #CSB-EQ027743MO) and anti-collagen type II antibody (anti-CII, Chondrex, USA, #2036) were determined using ELISA kits, following the manufacturer’s instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Saline, Two Tailed Test

Journal: Biology of Sex Differences

Article Title: Sex differences in disease severity and immune responses in murine and human inflammatory arthritis

doi: 10.1186/s13293-026-00840-w

Figure Lengend Snippet: Male CIA mice exhibit higher disease severity compared to females. Male and female CIA and saline control mice were monitored for disease severity and assigned clinical scores from day 21 after the first CII challenge until the end of experiment (day 29). (A) Line graphs representing the mean clinical scores of mice starting from day 1 to day 29. On day 29 after the first CII challenge, mice were euthanized by cardiac puncture under anesthesia for blood and serum collection and storage at −80 °C. Serum concentrations of (B) anti-mouse collagen type II antibodies (left panel) and anti-bovine collagen type II antibodies (right panel) were analyzed by ELISA. N = 5 mice per group. One of two independent experiments. Simple linear regression analysis was performed to determine the statistical difference between the lines. One-way analysis of variance (ANOVA) followed by Tukey’s multiple comparison post hoc test was used to determine the statistical significance between the groups. (*/ # p ≤ 0.05, ** p ≤ 0.005 and ***/ ### p ≤ 0.0005). In Fig. 1A, # significance of comparisons between CIA and saline control mice; *significance of comparisons between sexes of CIA mice

Article Snippet: Serum levels of mouse anti-collagen antibodies (autoantibodies) and bovine anti-collagen antibodies (antibodies to the immunizing antigen) were determined by ELISA using a Mouse Anti-mouse Type II Collagen IgG Antibody Assay Kit and Mouse Anti-Bovine Type II Collagen IgG Antibody Assay Kit, respectively, according to the manufacture’s protocol (Chondrex Inc. WA, USA).

Techniques: Saline, Control, Enzyme-linked Immunosorbent Assay, Comparison